Rationale

Today we will finalize our independent research question and explore the tools needed to answer the question.

Procedure

• A final question was created using a mixture of the questions proposed on 3/25/19.
• DNA Master and a variety of external tools were used to explore the %GC in NapoleonB.
• DNA Master was also used to look for repeated sequences in NapoleonB.

Results

The new research question devised explores the characterization of the coding regions versus the non-coding regions of AM cluster phages by comparing the %GC and the number of repeated sequences present.

Shown below is a picture of DNA Master and the %GC data for gene 1.

Conclusion/Next Steps

Next, we will determine the %GC of the non-coding regions and the coding regions of NapoleonB. We will also look for repeated sequences in the genome. After analyzing NapoleonB, we will expand our research to more AM cluster phages.