Purpose: The purpose of this lab is to finalize the independent research question and create a plan for the project and data collection.

Tools/Procedures:

Tools:

• Canvas
• DNA Master
• Online Bioinformatics Resources

Procedures:

1. A new question was formed from the list of questions created in the last lab. The new question focuses on characterization of the gaps of AM phages with %GC and repeated sequences, in comparison to coding regions.
2. Research was done to find the best ways to calculate %GC for each gene and gap.
3. Research was begun to look for ways to find repeated sequences in NapoleonB.
4. Work was saved.

Results: The results of this lab include creating a new research question and beginning the process of planning the project and data collection.

Picture: %GC

Conclusions:
In conclusion, the research question was created, centering around the characterization of AM phage gaps. Work was done to find the best way to collect the data and analyze the results.

Future Work:
Future work will include collecting the data for the research project and finding repeated sequences. Once data has been collected, the results will be analyzed.