Rationale:

The purpose of today’s lab was to correct the genes that were found to have errors in them, as well as finish a group submission poster for the class to review.

Tools:

• NapoleonB PhageNotes
• DNA Master
• PowerPoint

Procedure:

• Gene 64 was found to have a coding potential error, and a gap miscalculation. In addition to this, gene 61 was found to have an RBS value error.
• Both genes had their errors corrected, and a final presentation poster was completed and submitted as well.

Results:

• It was decided that gene 64 did not cover all of the coding potential marked by genemark and that it has an overlap of 11 instead of 9.
• Also, the Z-value for gene 61 was inputted incorrectly and was corrected to the proper 3.113 instead of 3.118.
• Final poster submission:
  • 

Conclusions:

It was decided to change the coding potential originally annotated for gene 64 as the atypical coding potential extended past where the gene was called. The gene could have been pulled back to a longer ORF, however that would have violated the overlap rule that guided phage genome annotations. The overlap miscalculation was an error on my part as I did not add, I subtracted. Also, the z-value was an input error.

Next Steps:

The next steps for this experiment are to decide on a poster to represent as a class and check my starterator hits.