February 27

Poster Design 2/25/19

Rationale: Design poster for Scholars Week.

Procedure: Previous SEA-PHAGE posters along with other posters were analyzed to gain insight on how to make a proper poster. The layout of the poster was designed as a sketch in a group of three. Reference posters, paper, and a pencil were used to help design a sketch of the possible poster that could be used to represent the class at URSA poster presentations.

Results: Poster design was done on a blank white sheet of paper. Group decided to leave out the abstract, and a sketch of the whole poster was done. Introduction, methods for both wet lab and in sillico, discussions, conclusions, and references were included.

Conclusion: Poster designs completed, but not filled with any flow charts or any bullet points. Colors of the poster were determined, and the overall ideas of what to add in the poster were established.

Future plans: Finish the poster designs and the layouts. Begin building the poster and to start self-group research questions.

February 27

February 27 2019 Poster Creation

Purpose: The purpose of this lab is to design and create a poster for Scholars Week.

Tools/Procedures:

Tools:

  • Microsoft Powerpoint
  • Lab Data
  • Abstract
  • Institutional Logos

Procedures:

  1. A powerpoint presentation was created and shared with the group.
  2. Powerpoint settings were created and an outline of the poster was made.
  3. Information was added to the poster.
  4. Figures were obtained or created and added to the poster.
  5. The poster was saved.

Results:
A rough draft of the final poster was created, and the data were inputted.

Conclusions:
The poster rough draft was finished, but the poster was not. The layout of the poster still needs to be adjusted and fixed. Also, the poster needs to be checked to see if more data from the lab needs to be added.

Future Work:
For future work, the poster needs to be finalized and all formatting issues need to be fixed. The final poster will be compared to other groups and used to create a final class poster presentation.

February 27

2.27.19 Creating PowerPoint Poster

2.27.19 Creating PowerPoint Poster

Rationale: After the paper sketch was returned, the next step was to transfer the ideas to a PowerPoint slide (the typical tool used to make scientific posters). Therefore, today was dedicated to beginning the process of creating a PowerPoint version of what had been established on Monday.

Procedure:

Sketch was analyzed and revised. Work was split up amongst the three group members  and different sections were created today. The sections were worked upon until the end of lab.

Results:

A PowerPoint slide with general content was created today. The time permitted was not nearly enough to compile all of the content needed for a complete poster, so only some of the content was present on the submitted copy.

Conclusions:

Creating a poster is very difficult to do in an hour and a half. The process is far longer and requires a lot of attention and planning. In the planning process, it would have been more beneficial to focus more on content and less on how the information would look on the slide. However, a good beginning to a slide was achieved today and will provide a foundation for future work.

Next Steps:

Information that was put on the slide today will need revision, and the rest of the information that had yet to be added will need to be analyzed and added during the next opportunity.

February 27

2.25.19 Sketching Poster

2.25.19 Sketching Poster

Rationale: Since a poster is the most common form of presentation for URSA, it was necessary to learn more about what successful posters look like and to begin to brainstorm what our own posters may look like eventually. Therefore, we looked at many past posters and were assigned into the groups we brainstormed our own posters in.

Procedure:

As a class, we reviewed many posters for coloring and content. We discussed things we liked and those we didn’t. Then, we were assigned into groups and in those groups, we discussed and planned important content we wanted to include on the posters. Finally, we created a visual representation on paper of what we would like our posters to look like.

Results:

This lab session resulted in a more complete understanding of what a research poster entails. In addition, this period also resulted in us developing a sketch about what our own posters would look like in small groups.

Conclusions:

Posters have far more moving parts than initially anticipated and take a lot of thought and effort. They are benefitted by careful planning and analysis before action, and many review processes are required before a final product can be submitted.

Next Steps:

On Wednesday, a PowerPoint version of the poster brainstormed will be created. This is the next step in beginning to create a poster; the product will need to be reviewed and changed many times before it can be fully submitted.

February 27

Making Visuals for Poster Rough Draft 2/27/19

Rationale: Since we determined good visuals are important for a good poster we will work on making aesthetically pleasing visuals.

Tools: Excel, Photoshop

Procedure:

  1. Took all the data from the past year of research and attempted to make a chart for each relevant piece of data.
  2. Made a sunburst chart to represent gene function calls, pie chart for soil composition and tree measurements
  3. Cropped photos from wet-lab results to make them more visually appealing.

Results: N/A

Conclusion and Future Work: The visuals definitely need more work to look as visually appealing as I was shooting for. Therefore, I will work some more to make sure that the information is provided in an aesthetic way.

February 26

February 25 2019 Poster Design

Purpose: The purpose of this lab is to begin designing posters for Scholars Week.

Tools/Procedures:

Tools:

  • Reference Posters
  • Paper, Pencil

Procedures:

  1. Previous SEA-PHAGE posters and other example posters were looked at and analyzed to gain insight into proper poster presentation and to see what makes a poster look good.
  2. The layout for the poster was designed.
  3. The most important figures to include on the poster were discussed.

Results:
The design for the poster was begun. It was decided that it was not necessary to include the full abstract on the poster, but to provide a summary abstract section with the introduction. The layout was created, and figures were chosen to be included on the poster.

Conclusion:
In conclusion, the poster design was not finished, but the process of designing was started. Discussions were begun about which figures would best represent the research done be visually pleasing to an audience.

Future Work:
Future work will include finishing the poster design and layout. The next step will to begin building the poster and adding the research.

February 25

Drawn Poster Rough Draft 2/25/19

Rationale: To prepare for the Scholar’s Day presentation we will begin rough drafting our poster by hand.

Tools: N/A

Procedure:

  1. Researched good poster making strategies and viewed posters from years prior.
  2. Drew a rough outline on paper of what we wish the poster to look like at the end.

Results: N/A

Conclusions and Future Work: We decided that symmetry and visuals are key parts of the poster we want to work on. We will begin working on the poster next time.

February 22

NapoleonB Annotations

2/20/19

Rational:

To check the annotations of NapoleonB in order to fix possible errors.

Procedure:

  • Looked for gaps and overlaps that could indicate a mistake
  • Rechecked GeneMark using the correct host
  • Checked the coding potential for genes 9-12 and 98
  • Checked the SCS for the genes

Observations:

  • The host that was originally used for GeneMark was inncorrect which gave incorrect information for coding potential
  • The answer for SCS was also changed for a few of the genes
  • The answers for coding potential did not change

Fig.5 – This image shows that the GeneMark results show coding potential for gene 10, but not a gene

Conclusion:

The errors that were found in the assigned genes as well as the rest of the genes were fixed. Next lab we will work on the poster for the lab.

February 22

Annotation of Gene 98

2/18/19

Rational:

To finish annotating gene 98. Also to write an abtract for the lab with my group using the abtracts we had written and brought to class.

Procedure:

  • Checked GeneMark to make sure the coding potential was covered
  • Entered the start and stop codons
  • Checked phagesdb to make sure the starterator agreed with the called start
  • BLASTed the gene in phagesdb and NBCI
  • Entered the information about the BLAST-Start from the hit from phagesdb and NBCI
  • Calculated the gap
  • Entered the information about the RBS for gene 98
  • Used NBCI, CDD, phagesdb, Synteny, and HHpred to find the gene’s function
  • Wrote the finl draft of the abtract that my group wrote

Observations:

  • The hit from phagesdb and NBCI was Mudcat
  • The calculated gap was 2
  • The SD score for RBS was not the best start
  • The genes function was HTH DNA binding domain protein

Fig.4 – The red number under the final score column shows the score for the chosen start, but the highlighted portion shows a start with a score closer to zero. This means that the current start is not the best according to RBS.

Conclusion:

Gene 98 was annotated nd it function was determined to be an HTH DNA binding domain protein. Next lab I will check the annotaions for errors before it finished.