Title: Checking NapoleonB’s Genes

Date: 20 February 2019

Rationale: Before the final annotations of NapoleonB are sent in, the genes must be checked for accuracy and any abnormalities double checked and justified.

Tools Used:

• DNA Master
• NCBI BLASTp
• PhagesDB BLAST
• GeneMark

Procedure:

• Genes 47 and 48 were checked to ensure that the chosen start was justified by BLASTing and comparing gene products. Other abnormal calls were also checked by the class and any mistakes fixed as observed.

Results/Observations: The results from NCBI and PhagesDB showed that the starts chosen (not the longest available ORF) were justified (due to q1:s1) as well as a worse blast hit when the reading frame was pulled back. No changes were made to the original calls.

Conclusions/Next Steps: Once a final abstract is selected, poster design will commence as well as possibly another check of the NapoleonB annotations to ensure accuracy. Individual research projects will also start soon.