2/18/19

Rational:

To finish annotating gene 98. Also to write an abtract for the lab with my group using the abtracts we had written and brought to class.

Procedure:

• Checked GeneMark to make sure the coding potential was covered
• Entered the start and stop codons
• Checked phagesdb to make sure the starterator agreed with the called start
• BLASTed the gene in phagesdb and NBCI
• Entered the information about the BLAST-Start from the hit from phagesdb and NBCI
• Calculated the gap
• Entered the information about the RBS for gene 98
• Used NBCI, CDD, phagesdb, Synteny, and HHpred to find the gene’s function
• Wrote the finl draft of the abtract that my group wrote

Observations:

• The hit from phagesdb and NBCI was Mudcat
• The calculated gap was 2
• The SD score for RBS was not the best start
• The genes function was HTH DNA binding domain protein

Fig.4 – The red number under the final score column shows the score for the chosen start, but the highlighted portion shows a start with a score closer to zero. This means that the current start is not the best according to RBS.

Conclusion:

Gene 98 was annotated nd it function was determined to be an HTH DNA binding domain protein. Next lab I will check the annotaions for errors before it finished.