Rationale:

To begin the genome analysis of phage NapoleonB, DNA Master has been chosen to be the software to annotate the genetic sequence. PhageNotes is also introduced as a convenient way to annotate genes and revising them. Abstract for the presentation was discussed, since an abstract should concisely inform readers or poster viewers the important points of the research.

Materials:

• Laptop
• DNA Mastering Program
• Gene sequence of phage NapoleonB

Procedure:

1. Some of the red flags on gene annotations were reviewed, gene 78 was examined since it has a 35 bp overlap with the previous gene.
2. Abstract draft was discussed and written in a small group and submitted at the end of the lab.

Results/Next Steps:

Abstract Draft:

Arthrobacter is a bacteria genus commonly found in soil and sewage that can be utilized in bioremediation and degradation of pesticides. The HHMI SEA-PHAGE initiative at Baylor University (BEARS in the SEA) used Arthrobacter sp. ATCC 21022 as a host to isolate Arthrobacter phage found around the Baylor campus. From over 60 soil samples collected, 8 were positive for Arthrobacter phage, six were submitted to PhagesDB, and novel Arthrobacter phage NapoleonB was sequenced. The genome of the bacteriophage was annotated using bioinformatic tools. Of the 100 predicted genes of Bacteriophage NapoleonB, 72 of the genes had no clear functionality. The predicted genes have been put into a database and used to cross-reference with other phages. Further research could be conducted in order to determine the functions of the unknown proteins.

The gene 78 call wasn’t changed in order to cover the coding potential in the overlapped region.

In the next lab, final gene annotations and abstract draft would still be the main objective.