Rationale

Today we aim to check the annotations of NapoleonB and come together in groups to make an abstract of the research conducted last semester and this semester.

Tools

• DNA Master
• PhagesDB
• NCBI
• Phamerator
• Genemark
• HHPred

Procedure

• An abstract draft was made by combining the stronger parts of other student’s abstracts in the group.
• The annotations for gene 96 was re-evaluated due to a large gap present between genes 95 and 96.
• Coding potential, and PhagesDB and NCBI blast hits were re-evaluated to determine whether or not the large gap should be closed.
• Phamerator was employed to compare the different regions to various AM cluster phages.

Results

The revised abstract was submitted. The large gap between genes 95 and 96 was deemed to possess a lack of need to be altered.

Conclusion/Next Steps

Next, we will finalize the abstract draft made. We will also look more closely at the full annotated genome of NapoleonB before submitting.