Abstract Refinement and Assigned Gene Corrections

Date: 2-18-19

• Rational
  • The rational for this lab is to work on refining our abstract drafts as well as to correct genes with questionable calls for NapoleonB.
• Procedure
  1. Groups were formed and abstracts between each member were compared.
  2. A consolidated version of the abstracts was created and submitted for further critique.
  3. The group was tasked with analyzing the calls made for gene 44.
  4. Phamerator, NCBI BLAST, and starterator, as well as the upstream gene – gene 43 – were studied to see whether gene 44 could be edited and called at a later start codon
  5. The gene was moved from a start codon at bp 30318 to a start codon at bp 30372.
• Results
  • NapoleonB gene 44 was shortened to a new start codon to avoid an overlap of 86 bp, which is too much of an overlap to be accurate according to data on previously annotated genomes.
• Future Plans
  • The next step is check the rest of the annotations to make sure they are accurate before submission. Also a final class abstract must be constructed from each group.