Reviewing Annotation and Poster Review 2/20/18

Rationale

The rationale behind these procedures is to ensure that all annotations on NapoleonB are correct and the abstract is prepared for Scholar’s Day.

Tools/Procedure

1. Several annotations were examed as a class to ensure correctness
2. Genes were reexamined using:
   • NCBI was used to BLAST amino acid sequences against a large database of recorded sequences
   • Starterator and genemark were used to determine if the genes ought to be altered to include more or fewer base pairs
3. The information gathered was used to verify the gene calls that were previously made
4. Poster requirements were reviewed to increase understanding

Results

Upon reviewing annotations a gene between genes 66 and 67 was called. It was discovered to have NKF and I will finish it annotations by Monday lab (see image above).

Conclusion

There is not a lot of information from which to draw conclusions, but it is interesting to see that there a gene in between 66 and 67 because there was no coding potential shown on genemark.

Future Plans

In the future, I will use the annotations of NapoleonB to be able to research my own questions and hopefully add in someway to knowledge about phage.