February
21
DNA Day 10
20 February 2019 ✷ NapoleonB DNA Annotation Corrections (again)
Rationale: NapoleonB’s genes were assigned to each student to annotate and record in order to complete the genome annotation for the phage. Annotations for several genes were called into question by the TAs and the class discussed the annotations as a class.
Procedure
- DNA master was opened and NapoleonB was auto-annotated
- PhageNotes was opened and each aspect of the DNA annotations for several genes was analyzed.
- Gene 23 was moved back 6 base pairs in order to generate a better q:s match in the databases.
- Abstracts for research performed last semester and this semester were crafted prior to lab and members of the group combined the best parts of each abstract into one strong one
- the poster for the group was somewhat planned (i.e. what should be included).
Results
Conclusion
Many of the genes annotated were no known function or tail proteins, so the study of the phage is largely centered around that.
Future plans
The annotation of NapoleonB’s genome will be completed, checked, and submitted to phagesdb. The information gathered from the annotation of the genome will lead to the creation of a research question and thus further research into phage biology involving NapoleonB.