02/18/19

Rationale: to acquire the fully annotated genome of Napoleon B

Procedure:

1. DNA master was opened and Napoleon B was opened in DNA Master.
2. Gene 44 was reevaluated
3. NCBI was used to blast the gene and acquire genes in the database that matched with the query gene, which was used to find the possible similarity and function of the query gene
4. Genemark was used to check the coding potential covered by the gene and the possible changes that could be made if possible to acquire the longest ORF possible.
5. Phagesdb was used to blast the gene and acquire genes in the database that belonged to other phages that matched with the query gene, which was used to find the possible similarity and function of the query gene
6. HHpred was used to predict the protein structure of the gene product, which was run through its database (Pfam and COG)for a possible matches with other proteins, which could be used to predict the function of the protein.
7. Phamerator was used to compare the genome of napoleonb to other phages in the same category to compare syntony of the genes.
8. phage notes was used for input of all the required information correcting the gene annotation.
9. After, the abstracts of group members were compared and one sample abstract was submitted.

Results:

Gene 44 annotation:

SSC:30372 – 30815, CP:Yes, SCS:Both, ST:SA, BLAST-Start:Aligns with Circum gp46 NCBI BLAST q44:s1 1 6E-106, Aligns with Circum gp46 PhagesDB BLAST q44:s2 1 9E-84, Gap:8bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 3.102 -3.355 No, F:NKF, SIF-BLAST:NKF, SIF-HHPred:NKF, SIF-Syn:NKF

Conclusion:

gene 44 has no known function.

Future steps

select abstract for scholars day submission