Purpose: The purpose of today’s lab is to re-check NapoleonB’s annotations and begin preparing for work on the Scholars Day poster.

Tools/Procedures:

Tools:

• DNA Master
• Phage Notes

Procedures:

1. Annotations for NapoleonB were uploaded to DNA Master.
2. Errors in start and stop positions were fixed and all genes were checked for correct length and position.
3. Gene 1 and 2 were looked at, and it was determined that a reverse gene could not be added to replace gene 1. It is still uncertain if gene 1b will be added.
4. Poster group work was started.

Results:
All of the genes for NapoleonB were checked again and looked at as a class. This helped to determine if the correct starts were chosen for each gene. It is still uncertain whether or not gene 1b will be added, since it is a reverse gene in between two forward genes.

Conclusions:
In conclusion, NapoleonB’s genome is still being checked. Checking the annotations multiple times will allow for more accurate results and for errors to be caught and corrected. Poster collaboration was begun and will be continued throughout the next few labs.

Future Work:
Future work will include finalizing NapoleonB annotations and completing a poster to present at Scholars Week.