2.20.19 Checking NapoleonB – Part 2

Rationale: Since part of the annotation process is checking work, it is necessary to return to a complete annotation with fresh eyes and examine it again. Therefore, the class looked over the majority of the genes in NapoleonB that had been called once more to check to ensure annotations were properly done.

Tools/Procedure:

• Genemark
• DNA Master
• NCBI BLAST
• PhagesDB Blast

1. Opened PhageNotes and DNA Master
2. Checked and discussed each gene that was questionable
3. Ran a separate Genemark with the correct species

Results:

The time in the lab today resulted in portions of calls being changed for a few genes that had been mistyped or had errors with the calls. The class had failed to use the proper species when using Genemark for the first time, so the correct species was used and some results were changed because of this.

Conclusions:

The main conclusion from today’s lab time was that the species is significant in the Genemark system. This was a previously forgotten fact, and it would have been detrimental to the annotations if it had gone unnoticed. Furthermore, today proved that one check was not enough, as the class found more discrepancies that were missed the first time around.

Next Steps:

During the next lab time, the independent projects will be examined more closely along with poster & abstract ideas for Scholar’s Week. Furthermore, it would be beneficial to examine NapoleonB with more detail than was done today.