February 20

2.20.18 Checking NapoleonB Annotations

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Rationale:

To check the annotations of NapoleonB as a class in order to catch any mistakes that were made.

Procedure:

  1. Using an already auto annotated version of NapoleonB on DNA Master gene 18 was confirmed to have the correct LORF.
  2. Synteny was also updated for genes 19 and 20.
  3. The rest of the genome was proofed as a class.

Results:

The synteny for genes 19 and 20 were updated using the phage KeaneyLin in addition to Nason. The rest of the genome was moderately corrected in order to fix entry errors.

Conclusions:

It can be confirmed that gene 18 was correct in its annotation. It can also now be concluded that genes 18, 19, and 20 do have synteny as shown in phamerator. It can also be concluded that the annotations for the phage NapoleonB are as of now correct.

Next Steps:

The next step will be to conduct an independent research project. We will also create a poster as a group in order to present at scholars week as a class, along with finalizing a class abstract.


Posted February 20, 2019 by nathan_newton1 in category Nathan Newton

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