NapoleonB Gene 26 and Abstracts (2/18/19)
Rationale:
Corrected NapoleonB gene 26 and combined abstracts from other group members for a possible poster abstract.
Tools:
- DNA Master
- NCBI
- PhagesDB
- GeneMark
- HHpred
- phage notes
Procedure:
- Opened DNA Master and phage notes.
- Checked for mistakes in NapoleonB gene 26 with GeneMark, “Choose ORF start” window in DNA Master, NCBI, PhagesDB, and HHpred.
- Corrected the start coordinates.
Results:
The picture below shows the corrected annotation for NapoleonB gene 26.
Conclusion:
The start coordinates were corrected to 24802 bp. Cooper’s and Lily’s introductory sentences, Nathan’s methods part, and my results sections were combined into an abstract. Working in groups can be a great tool when brainstorming ideas for a presentation; however, groups are not as effective when it comes to writing. One interesting finding placed in the abstract was that NapoleonB is the only AM clustered phage to have a gene with the holin function. This gene might be the reason why NapoleonB had an extremely high titer relatively fast.
Future Work:
Start individual research projects and design a poster to show at conferences.
