Purpose: The purpose of this lab is to check the annotations for NapoleonB and write abstracts for Scholars Week.

Tools/Procedures:

Tools:

• DNA Master
• NCBI
• PhagesDB
• HHpred
• Phamerator

Procedures

1. The gap between genes 1 and 2 for NapoleonB was analyzed and it was determined that a reverse gene should be inserted.
2. Annotations for gene 1B were completed and added to PhageNotes.
3. Annotations for other genes were checked and corrections were made if necessary.
4. Abstract for Scholars Week was edited and submitted.

Results:
Gene 1B was added to NapoleonB. This gene, while it is a reverse gene in a forward area, is common to many other AM cluster phages.

Annotation for Gene 1B:

SSC:372 – 569, CP:No No coding potential was present, SCS:Neither, ST:NA, BLAST-Start:Aligns with circum gp2 NCBI BLAST q1:s1 100 2E-41, Aligns with Tribby gp2 PhagesDB BLAST q1:s1 1 6E-35, Gap:first gene, LO:Yes, RBS:Kibbler7 and Karlin Medium 3.263 -2.059 Yes, F:NKF, SIF-BLAST:NKF, SIF-HHPred:NKF, SIF-Syn:NKF

Conclusions:
In conclusion, gene 1B was added to PhageNotes and annotated were complete. The other assigned annotations were checked to make sure they are correct and also updated on PhageNotes. The group abstract for Scholars Week was edited and completed. At this point, all of the gene annotations should have been checked.

Future Work:
Future work will include continuing to check NapoleonB’s annotations, as they need to all be correct. Also, we will continue to prepare for Scholars Week and create the poster.