Rationale: Continue annotating assigned genes of NapoleonB

Process: Used DNA Master, GeneMark, NCBi Blast, NCBi cd, phagesdb, and HHPRED to annotate gene 82-84 of NapoleonB

• changed the start codon of gene 83 to include more coding potential
• no evidence of any function for all three genes

Result:

Gene 82:

SSC:48102 – 48746, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Arthrobacter phage KeaneyLin gp77 NCBI BLAST q1:s1 0.99 2E-145, Aligns with Arthobacter Phage KeaneyLin gp77 PhagesDB BLAST q1:s1 0.96 1E-114, Gap:27bp gap, LO:Yes, RBS:Kibbler7 and Karlin Medium 3.263 -2.348 Yes, F:NKF, SIF-BLAST:NKF, SIF-HHPred:NKF, SIF-Syn:NKF

Gene 83:

SSC:48743 – 48818, CP:Yes, SCS:BothGM, ST:SS, BLAST-Start:Aligns with Arhtrobacter Phage Circum gp82 NCBI BLAST q1:s1 0.76 1E-32, Aligns with Arhtobacter Phage Circum gp82 PhagesDB BLAST q1:s1 76%% 2E-28, Gap:4bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 2.188 -4.267 Yes, F:NKF, SIF-BLAST:NKF, SIF-HHPred:NKF, SIF-Syn:NKF

Gene 84:

SSC:49015 – 49233, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Arthrobacter phage Cheesy gp84 NCBI BLAST q1:s1 0.98 4E-45, Aligns with Arthrobacter Phage Cheesy gp84 PhagesDB BLAST q1:s1 0.98 3E-36, Gap:65bp gap, LO:, RBS:Kibbler7 and Karlin Medium 1.98 -4.847 No, F:NKF, SIF-BLAST:NKF Supported by NCBI BLAST gp , NKF Supported by PhagesDB BLAST gp , , SIF-HHPred:NKF, SIF-Syn:NKF, upstream gene is , downstream gene is , just like in phage

Next Steps: continue annotating NapoleonB genes