Title: NapoleonB Annotation

Date: 13 February 2019

Rationale: The annotations for NapoleonB will be finished and reviewed for accuracy as well as the starterator (ST) annotation completed

Tools:
– Microsoft Surface Pro 5 Tablet
– DNA Master Software
– NCBI BLASTp tool
– HHPred tool
– GeneMark
– PhagesDB BLAST
– Phamerator

– Starterator (PhagesDB)

Procedure: Using the above tools, gene 95 were annotated completely
and the starterator annotation completed for genes 45, 47, and 48.

For gene 95:
– A start codon call was made based on the Glimmer/GeneMark call
– The product sequence was BLAST’ed to determine any present function
– A function no-call was made

Results/Observations: The following picture contains the results of the
annotation for gene 95:

Conclusions/Next Steps: The starterator annotations were finished, giving insight into what information the other phages in the AM cluster revealed about a specific gene’s start. With the exception of one more final review, the genes in NapoleonB have, for the most part, been completely annotated. They will likely be reviewed and any changes made before the analyzation begins on the genome as a whole.