FEBUARY 11TH, 2019 

• OBJECTIVE: 
  • Annotate assigned Napoleon B genes 
• PROCEDURE
  • FasA file of Napoleon B was run through gene mark, and was downloaded into DNA Master 
  • The file in DNA master was auto-annotated
  • Genemark was analyzed to determine the start of gene 5
• Phages DB was used to determine the protein function, and was used to determine the relation to phage Tribby 
• RESULTS: 
  • Gene 5 

SSC:2458 – 2826, CP:Yes, SCS:Both, ST:NI, BLAST-Start:Aligns with  gp NCBI BLAST q:s  , Aligns with Tribby gp6 PhagesDB BLAST q1:s1 1 6E-67, Gap:25bp gap, LO:Yes, RBS:Kibbler7 and Karlin Medium 2.869 -3.568 No, F:NKF, SIF-BLAST:NKF Supported by PhagesDB BLAST Tribby gp6   , , SIF-HHPred:NKF, SIF-Syn:NKF

• CONCLUSION:
  • Gene 5 is partially annotated and needs to be finished
• FUTURE STEPS: 
  • Finish annotating gene 5, and the 3 other assigned genes 
• FEBRUARY 13TH, 2019
• ABSENT