Rationale

Today we will finish the annotations assigned for the genome of NapoleonB.

Tools

• NCBI
• HHPred
• Phamerator
• PhagesDB
• Genemark
• DNA Master
• Phage Notes

Procedure

• DNA master was opened and NapoleonB’s FASTA file was autoannotated.
• The protein sequences were run through NCBI Blast, HHPred, and PhageDB.
• The remaining tools were utilized to complete the rest of the annotations of Phage Notes. Phamerator was utilized to determine the synteny, while genemark displayed the coding potential.

Results

The results for genes 86-88 are shown below.

Conclusions/Next Steps

Next, we will ensure that no errors were detected in the annotated genome. The annotations will then be submitted to PhagesDB.