Rationale:

The purpose of the lab was to start annotations of NapoleonB’s genes.  

Tools: 

• DNA Master 
• PhagesDB  
• NCBI BLAST 
• Phamerator 
• HHPred 
• GeneMark  
• PhageNotes 

Procedure:  

1. Genes were assigned for phage NapoleonB annotation.  
2. The FASTA file was downloaded and opened with DNA Master.  
3. NapoleonB was autoannotated and the ORF of gene 13 was located.  
4. Gene 13 was annotated on PhageNotes and used information from PhagesDB, NCBI Blast, Phamerator, HHPred, GeneMark,  

Results: 

Gene 13 of NapoleonB had no known function.  

Gene 13 Database Results:

Annotation of Gene 13:

Conclusion: 

Annotation seemed easier with more practice. The start value of gene 13 was not changed and was determined with the support of PhagesDB, HHPred, and NCBI Blast, since gene 13 overlapped with gene 12. The E-value and the coding potential for gene 13 seemed to be better when the start was not changed.  

Future Work :

Annotations of Gene 14,15,16, and 96 will be completed in the future labs.