Rationale

Today we started annotating the genome of NapoleonB.

Tools

• DNA Master
• NCBI Blast
• HHPred
• PhagesDB
• Genemark
• Phamerator
• PhageNotes

Procedure

• NapoleonB’s FASTA file was downloaded and opened in DNA Master.
• The genome as auto-annotated.
• Gene 85 was located and DNA Master, NCBI Blast, HHPred, and PhagesDB were utilized to help annotate the genome on PhageNotes. Genemark and Phamerator were utilized as well.

Results

The results of the final annotation of gene 85 is shown below. The function of gene 85 was reported to be HNH endonuclease.

Conclusion/Next Steps

Next, the annotations of genes 86-88 will be conducted to finish the assigned genes of Napoleon B. The annotations will allow for further research on NapoleonB,