Rationale: Continue annotating the genome of NapoleonB, specifically assigned genes 37-40.

Materials:

• DNA Mastering
• NapoleonB PhageNotes
• PhagesDB
• NCBI Database
• Phamerator
• GeneMark

Procedure:

• Booted up DNA Mastering on computer and opened NapoleonB file
• Continued annotating NapoleonB genes 37-40
• After completing, worked on the QTM due that lab day (Number 9)
• Utilized PhagesDB and NCBI to check protein sequences, and Phamerator to check predicted shape

Observations:

Complete annotated genes of 37-40 for NapoleonB

Questions I had on QTM 9, such as which genes I had problem with

Conclusion/Next Steps: After complete annotation, it’s expected that the genes will be reviewed and checked for correctness. After that process, will continue to work with NapoleonB and create scientific questions to be answered.