February
14
DNA Day 8
13 February 2019 ✷ NapoleonB DNA Annotation pt 2
Rationale: NapoleonB’s genes were assigned to each student to annotate and record in order to complete the genome annotation for the phage. Annotations for genes 21-24 and 97 were started and completed.
Procedure
- DNA master was opened and NapoleonB was auto-annotated
- PhageNotes was opened and each aspect of the DNA annotations for genes 22-24 was entered into the program and genes 22-24 and 97 were annotated to completion.
- Blasts for products (protein) were run on PhagesDB, HHPred, and NCBI to ensure accurate and up-to-date blast information.
- Phamerator was opened for AM phages and the synteny of NapoleonB was analyzed compared to genomes of phages in the AM group.
- From PhagesDB, starterator for each gene was opened and compared and all genes annotated agreed with starterator.
- The information was recorded and saved in the google sheet.
Results
the complete annotations for genes 22-24 and 97 as entered into PhageNotes, are as follows:
Conclusion
In genes 22 and 23, there was uncertainty in the function of the gene. The databases couldn’t agree or provide a function, thus it was decided that the function was unknown, or NKF.
Future plans
The annotation of NapoleonB’s genome will be completed, checked, and submitted to phagesdb. The information gathered from the annotation of the genome will lead to the creation of a research question and thus further research into phage biology involving NapoleonB.