Rationale: Annotate genes assigned on Arthrobacter NapoleonB

Materials:

• DNA Mastering
• PhagesDB Blast
• NCBI Blast
• Starterator
• NCBI Domains Website
• Computer

Procedure:

• Opened up DNA Mastering on computer
• Downloaded NapoleonB file from PhagesDB database
• Opened the file on DNA Mastering and auto-annotated
• Was assigned to annotate genes 37-40, placing results on NapoleonB PhageNotes
• After working on annotations, saved annotated NapoleonB file

Observations:

Genes of NapoleonB!

NCBI Blast results of gene 39

The unfinished annotations of genes 37-40 of Arthrobacter NapoleonB

Conclusion/Next Steps:

• It’s very exciting to finally start annotating Arthrobacter phage NapoleonB! Although the day was filled with confusion and a little but of chaos, the annotations are on their way to being finished. The next steps would be to continue annotating the genes and then have them be checked.