Rationale: Begin annotations for NapoleonB genes 41-44. Use knowledge from past few practice annotations to start NapoleonB annotations.

Procedure: NapoleonB FASTa file downloaded from PhageDB, and loaded onto DNA Master. Gene auto-annotated and frames were viewed. NCBI, HHPred, PhageDB, and Phamerator were all pulled up to annotate gene 41. The product of Gene 41 was copied and pasted onto  NCBI, HHPred, PhageDB, and Phamerator. Results were analyzed and annotations were placed into PhageNotes.

Results: 

Gene 41 annotation below followed by NCBI, HHPred, GeneMark, and PhageDB:

SSC: 29246 – 29362, CP: Yes, SCS: Both, ST:SS, BLAST-Start: Aligns with Mudcat gp39 NCBI BLAST q1:s1 2E-18, Aligns with Xenomorph gp38 PhagesDB BLAST q1:s1 1E-14, Gap:2bp gap, LO:N/A, RBS: Kibbler7 and Karlin Medium 3.012 -2.606 Yes, F:NKF, SIF-BLAST: NKF, SIF-HHPred: NKF, SIF-Syn:NKF.

Conclusions: Annotation of gene 41 had no known functions.

Future plans: Continue with the annotation of NapoleonB gene 42.