Rationale:

To annotate gene 17 from the phage NapoleonB.

Procedure:

1. DNA Master was opened with the fasta file for NapoleonB being selected.
2. NapoleonB’s sequence was auto-annotated.
3. The ORF screen was opened and the RBS values were opened as well.
4. Gene 17 was then annotated.

Results:

Gene 17

SSC:12925 – 14769, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Tribby gp18 NCBI BLAST q1:s1 1 0, Aligns with Tribby gp18 PhagesDB BLAST q:s 0, Gap:1bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 2.486 -4.688 No, F:major tail protein, SIF-BLAST:Tail Protein Supported by NCBI BLAST Tribby gpNA ASR80469.1 1 0, Tail Protein Supported by PhagesDB BLAST Tribby gp18 100 0, , SIF-HHPred:NKF, SIF-Syn:major tail protein, upstream gene is NKF, downstream gene is minor tail protein, just like in phage Nason

Conclusions:

It can be concluded that gene 17 is a major tail protein, as well as identical to the major tail protein found in the phage Tribby.

Next Steps:

The next step will be to annotate genes 18-20 of the phage NapoleonB.