Rationale:

The purpose of today’s lab was to get familiar with other databases which provide further evidence of the function of the assigned gene. Along with this, databases which displayed the location of tRNA was introduced as another piece of evidence. The complete annotation of gene 46 and 47 for phage Elesar was completed.

Tools:

• Personal Computational Device
• DNA Master
• HHPred
• NCBI
• PhagesDB

Results:

• After the introduction of the databases for tRNA, PhageNotes was introduced as a program to create a complete annotation of all the data was put in.
• Instructions were given to put in all the information for the assigned genes and to fill in any information was that was not placed.
• When the annotation was checked, several changes were made to the gene in the span of two days which resulted in changes made to the RBS of the previous annotation.

Conclusions:

After the lesson of the databases that were used for tRNA, instructions were given to complete the assigned annotations and to put the values into PhageNotes.

Future Plans:

Further introductions to more databases will be given in order to create the best annotation for Napoleon B.