Rationale

Today we will continue to annotate the genes 42 and 43 of Elesar for practice.

Procedure

• Genes 42 and 43 were previously annotated, however, corrections were made accordingly.
• DNA Master was opened and the protein sequence for genes 42 and 43 was obtained.
• The protein sequence was run through NCBI Blast, as well as HHPred and Gene Mark.

Results

Shown below are the NCBI Blast results from genes 42 and 43 respectively.

Shown below are the HHPred results from genes 42 and 43 respectively.

Final Annotation for Gene 42: SSC: 31255, 31935 CP: yes SCS: both-cs BLAST-Start: hypothetical protein PBI_MAJA_36, 36, NCBI, q46:s2, 2e-46 Gap: 3 overlap LO: N/A RBS: Kibler 7, Karlin Medium, 2.325, -3.883, no SIF-BLAST: NKF SIF-HHPred: Mitoribosomal protein mL52 , HHPred, n/a, PF18699.1, 47.9, 32.7 SIF-Syn: NKF

Final Annotation for Gene 43: SSC: 31932, 32198 CP: no SCS: Both BLAST-Start: hypothetical protein PBI_RYAN_46, 46, NCBI, q1:s1, 2e-53 Gap: 3 overlap LO: N/A RBS: [Kibler 7, Karlin Medium, 2.214, -4.470, no] SIF-BLAST: NKF SIF-HHPred: Secretin_N ; Bacterial type II/III secretion system short domain, HHPred, n/a, PF03958.17, 73.2, 86.81 SIF-Syn: NKF

Conclusion/Next Steps

Some parts of the gene annotations are still missing, however, the additions will be made at a later date. Once all the skills are learned to fully annotate a gene, we will move on to the annotation of NapoleonB.