Rationale: correct elesar annotations from the previous class, enter it into elesar annotation class spreadsheet, ans check a classmates annotations

Process:
1. reexamined the the RBS data to correct mistake

2. used NCBI BLAST to get data in addition to phagesdb BLAST

3. used CDD from NCBI to determine SIF syn

4. Entered data into spreadsheet

5. Checked Shepard Saabye’s annoation

Results:

• Gene 6: SSC:3685 – 4974, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Nandita gp7 NCBI BLAST q1:s1 0.99 0, Aligns with Nandita gp7 PhagesDB BLAST q1:s1 0.97 0, Gap:16bp gap, LO:Yes, RBS:Kibbler7 and Karlin Medium 3.215 -2.059 Yes, F:portal protein, SIF-BLAST:portal protein Supported by NCBI BLAST Nandita gp7 AYN58629.1 0.95 0, portal protein Supported by PhagesDB BLAST Nandita gp7 MH834621 0.97 0, , SIF-HHPred:portal protein supported by COG gpNA COG4695 0.86 , SIF-Syn:portal protein, upstream gene is terminase, downstream gene is capsid maturation protease, just like in phage Nandita
• Gene 7: SSC:4974 – 6221, CP:Yes, SCS:Both, ST:SS, BLAST-Start:Aligns with Nandita gp8 NCBI BLAST q5:s1 0.98 0, Aligns with Nandita gp8 PhagesDB BLAST q1:s14 0.93 0, Gap:1bp overlap, LO:NA, RBS:Kibbler7 and Karlin Medium 1.979 -4.696 No, F:capsid maturation protease, SIF-BLAST:capsid maturation protease Supported by NCBI BLAST Nandita gp8 AYN58630.1 0.89 0, capsid maturation protease Supported by PhagesDB BLAST Nandita gp8 MH834621 0.93 0, Caseinolytic protease supported by a CD found from the CDSEARCH databse NA gpNA cd07016 NA 2.98E-67, , SIF-HHPred:capsid portal protein supported by COG gpNA COG5518 0.91 , SIF-Syn:capsid maturation protease, upstream gene is portal protein, downstream gene is NFK, just like in phage Nandita

Future Steps:

Start annotating NapoleanB!