February 8

Elesar Annotations 2/4/2019

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Rationale: correct elesar annotations from the previous class and complete supporting information

Process:
1. reexamined the gap, RBS data, LO to correct mistakes

2. used HHPred to analyze predicted functions of the gene for supporting information

3. used phagesdb blast for the same purpose

4. Completed Annotation (Results):

  • Gene 6: SSC: 3685-4974 CP: yes SCS: both ST: NA BLAST-Start: matches with Nandita, 7, phagesdb, q1:s1, 97%, 0.0 Gap: 16 LO: yes RBS: Kibler7, Karlin Medium, 3.215, -2.059, yes F: portal protein SIF-BLAST: portal protein, phagesdb, Nandita_7, MH834621, 97%, 0.0 SIF-HHPred: Phage Portal Protein BeeE, COG, BeeE, COG4695, 86%, 99.89 SIF-Syn
  • Gene 7: SSC: 4974-6221 CP: yes SCS: both ST: NA BLAST-Start: matches with Nandita, 8, phagesdb, q1:s14, 93%, 0.0 Gap: 1 bp overlap LO: no RBS: Kibler7, Karlin Medium, 1.823, -6.049, yes F: capsid maturation protease SIF-BLAST: capsid maturation protease, phagesdb, Nandita_8, MH834621, 93%, 0.0 SIF-HHPred: Bacteriophage capsid portal protein , COG, NA, COG5518, 91%, 99.8 SIF-Syn

Future Steps:

Figure out how to use SIF syn and fix any new mistakes

 

 


Posted February 8, 2019 by rachel_melone1 in category Rachel Melone

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