Rationale:

The purpose of the lab was to correct any errors in the genes, and to continue with the remaining annotations of gene 44 and 45.  

Tools: 

• DNA Master 
• NCBI Blast  
• HHPred  
• Gene Mark 

Procedure: 

1. Corrections were made to the annotation of Elesar Gene 44 and 45.  
2. Gene Mark was used to obtain the results for the coding potential of the gene.  
3. DNA Master was opened, and protein sequences were obtained and used in HHPred.  
4. The databases for HHPred were changed according to our phage, and the results were noted.  
5. The genes were blasted on NCBI to obtain the percent alignment. 
6. The annotation was saved to be continued next time.

Results:  

Annotation of Gene 44:  

SSC: 32186, 32380 CP: yes SCS: both BLAST-Start: hypothetical protein PBI_NANDITA_47, 47, NCBI, Q1: S1, 4×10^-23  Gap: 0 LO: N/A RBS: Kibler7, Karlin Medium, 2.942, -2.636, yes SIF-BLAST: NKF, NCBI Blast, Arthrobacter phage Nandita 47, AYN58666.1, 83.33%, 4×10^23. SIF-HHPred : NKF, HHpred, N/A,PF11239.8, 83.33%, 0.65 

Annotation of Gene 45:  

SSC: 32373, 32909 CP: no SCS: both BLAST-Start: hypothetical protein PBI_RYAN_48 , 48, NCBI, Q1: S1, 1×10^-115 Gap: 0 LO: N/A RBS: Kibler7, Karlin Medium, 3.060, -2.325, yes. SIF-BLAST : NFK, NCBI Blast, Arthrobacter phage Ryan 48, AYN59038.1, 54.18%,  1×10^-115 SIF-HHPred : Pertussis toxin S5 subunit, N/A ,PF092.76, 54.18%, 62.95 

Conclusion:

Annotation practice on Elesar Gene 44 and 45 helped to better the understanding of how to annotate. Since HHPred, NCBI, and PhagesDB displayed no known function, it can be concluded that there is no known function for both Gene 44 and Gene 45.

Future Work:

In the future, gene 44 and 45 will be completely annotated and reviewed.  Using this knowledge obtained from Elesar Gene annotations, the process to annotate Napoleon B will be started.