Title: Annotation of Phage Elesar

Date: 4 February 2019

Rationale: After learning how to operate and use HHPred, an almost-complete annotation of phage Elesar can be made and posted to DNA Master.

Tools: 

• Microsoft Surface Pro 5 Tablet
• DNA Master Computer Software
• HHPred Bioinformatics Tool

Procedure:

• Started DNA Master and loaded previous file “ExtractedFromFastALibraryElesar.fasta”
• Opened HHPred Bioinformatics Toolkit on a different tab
• Due to no significant BLAST hit for genes 16 and 17, the SIF-BLAST annotations were both marked as “NKF”
• The product sequences for genes 16 and 17 were loaded into the input box of HHPred
• Due to no significant HHPred results, the SIF-HHPred annotations were both marked as “NKF”

Results/Observations:

• Below is a screenshot of the HHPred results for gene 16, showing the <90% probability and e-value of 39:

• Below is a screenshot of the HHPred results for gene 17, showing a >90% probability but an e-value of 0.12, which is not an acceptable value:

• Below is a screenshot of the final annotations entered for genes 16 and 17:
• 

Conclusions/Next Steps: In order to finally finish the annotation, the Starterator and Synteny entries must be made, but first the skills must be learned. Once the training annotations are finally completed, the necessary skills will have been learned to finally move onto annotating NapoleonB’s genome.