February
7
Phage Notes and Elesar Genes 2 & 3 (2/6/19)
Rationale:
Become familiar with phage notes, a Google spread sheet form designed to lower the number of mistakes when filling out the SEA-PHAGES template, and to peer check each other’s genes to further lessen the number of mistakes.
Tools:
- DNA Master
- NCBI
- PhagesDB
- GeneMark
- HHPred
- phage notes
Procedure:
- Copied and pasted Elesar genes 54 and 56 into phage notes.
- Performed an HHPred search on Elesar genes 2 and 3.
- BLAST was performed on Elesar genes 2 and 3 through both NCBI and PhagesDB.
- Conducted a GeneMark on Elesar.
- Annotated Elesar genes 2 and 3.
- Compared Elesar genes 2 and 3 annotations with peer’s.
Results:
The following image shows Elesar genes 54 and 56 annotations filled into phage notes.
The following image shows the checked Elesar genes 2 and 3 annotations in phage notes.
The annotations were completed except for the starterator section.
Conclusion:
Both phage notes and peer checking lowers the number of mistakes in the template.
Future Work:
Start annotating NapoleonB.
