February
7
DNA Day 6
6 February 2019 ✷ DNA Annotation (cont.)
Rationale: Elesar genes 2&3 were annotated and entered into Phagenotes
in order to practice for Napoleon B.
Procedure
- DNA master was opened and Elesar was auto-annotated
- Phagenotes was opened and each aspect of the DNA annotation for genes 2 and 3 were entered into the program; blasts were run again to ensure accurate and up-to-date information.
- Genes 2 and 3 were updated and annotations were checked by another student to verify accurate information.
- Genes 18 and 19 were also checked in order to verify the quality and accuracy of annotations.
Results
the updated annotations for each gene, entered into phagenotes, are as follows:
Conclusion
Programs like PhageNotes are helpful in annotating genes because coding the annotations is a very specific process. Simple issues from incorrect formatting can result in an annotation being rejected, so becoming familiar with PhageNotes will help improve the quality of annotations.
Future plans
This same method will be applied to Napoleon B, a phage isolated in 2018, in order to analyze its genome.