2.06.19- Inputting Annotations to PhageNotes & Checking Annotations

Rationale:

• In order to organize the information that had been collected over the previous lab sessions, Lathan created PhageNotes to efficiently compile the data into a usable format. Therefore, it was necessary for everyone to submit their gene annotations to the system. Furthermore, it was found to be beneficial to check each other’s genes to ensure that there were few errors.

Tools:

• DNA Master
• PhagesDB BLAST
• NCBI BLAST
• HHPred
• Genemark

Procedure:

• Opened DNA Master
• Opened files containing previous annotations
• Checked over all annotations and numbers using Genemark, BLAST softwares, and HHPred
• Inputted information into PhageNotes
• Checked PhageNotes & ran annotations for both Genes 10 & 11 to ensure proper notations had been found

Results:

• Gene 11’s LO was changed, as the database listed it as no when it was the longest possible. The rest of the genes appeared to be correct upon examination.
• Genes 50 & 51 were inputted to the database with slight alterations on gene 50’s HHPred, as certain fields should have been omitted and the changes were reflected in the official PhageNotes.

Conclusions:

• The genes were inputted to PhageNotes. Assuming there are no errors that had been missed, the PhageNotes will be able to be used to fill in the DNA Master with correct annotations. Furthermore, the script from DNA Master would be able to be sent to SEA-Phages for processing in the format it is in.

Next Steps:

• The techniques and skills will be applied to NapoleonB shortly.