Rationale: In order to practice more annotation and to help out others in the group we are proofreading each others annotations. I will be proofreading the annotation for genes 6 and 7. I will also learn how to use starterator and phamerator.

Tools: NCBI BLASTp, HHPred, PhagesDB, Phamerator, DNAMaster, PhageNotes

Procedure:

1. Uploaded the existing annotation for genes 28 and 29 to PhageNotes.
2. Annotated genes 6 and 7 myself using the template on the SEA-PHAGES website. I blasted the  genes and used HHPred to support my function calls.
3. Compared the annotation I made to the annotation for the genes in PhageNotes and told my partner about corrections that needed to be made.
4. Used Phamerator to look at the genes in NapoleonB and looked at the Starterator of a sample gene in NapoleonB.

Results:

Phamerator for NapoleonB and 2 other AM cluster phages

Starterator for NapoleonB gene 60

Conclusion and Future Work: Now that I’ve learned all the techniques for annotating genes I can start working on NapoleonB’s genome. PhageNotes is also a very useful tool for annotating as it makes it much easier to pool our work into one spot then copy over our annotations afterwords.