Rationale

Re-edit template from last lab day and add SIF-Blast and SIF-HHPred

Procedure

1. Opened DNA master with elesar fasta file
2. Opened DNA frames, clicked on ORFs, highlighted target gene, clicked on RBS
3. Blasted gene 4 via phagedb, NCBI, and HHPred
4. Edited template for SSC, CP, SCS, Blast-start, Gap, LO, and RBS and used guidelines and annotation key for assistance
5. Added SIF-BLAST and SIF-HHPred template
6. Repeated same procedures above for gene 5.
7. Clicked post after each gene annotation

Observations

By looking through NCBI, phagesdb, and HHPred, all of them seem to agree that the protein coded is a terminase small/large subunit, and the phage name is Nandita.

Next Steps

Have a better understanding of the templates and hope to correct any mistakes from today’s template.