Rationale: I’m continuing to practice annotation by annotating certain genes of the Elesar genome. Today I will be learning and practicing using HHPred and BLAST to discover the function of the gene.

Tools: BLAST, HHPred, DNAMaster

Procedure:

1. Opened up the DNAMaster file from last lab time containing the incomplete annotations for  genes 28 and  29.
2. Copied the amino acid product for each gene and ran it through HHpred using the COG_KOG_v1.0 and Pfam-A_v32.0 databases.
3. Recorded the results under SIF-HHPred. Gene 29 did not get a significant hit so I recorded it as No Known Function(NKF).
4. Next I ran the amino acid product through NCBI BLASTp and recorded those results under SIF-BLAST.
5. Both of the genes returned to be Minor Tail Proteins so I recorded that in my annotation.

Results:

HHPred and BLASTp results for genes 28 and 29.

Conclusion and Future Work: I learned how to use HHPred and determine the function of a gene. Next I will learn how to use synteny to support my call for the function as well as supporting my start choice with the starterator.