February
1
JAN 28TH AND 30TH- LABS
- JANUARY 28TH, 2019
- OBJECTIVE:
- Alter Annotation settings
- PROCEDURE:
- The description settings were copied from a Canvas page
- Those setting were then copied and pasted into DNA Master
- RESULTS:
- The settings were updated, and it now allows for the notes section to contain each part that must be completed for annotation (as seen in the figure below)
- CONCLUSION:
- Settings were successfully updated
- FUTURE STEPS:
- Start to annotate Elear’s genome
- JANUARY 30TH, 2019
- OBJECTIVE:
- Learn how to annotate genes, and attempt to successfully annotate genes 8 and 9
- PROCEDURE:
- DNA master was used to view and annotate genes
- Phages DB was used to BLAST genes
- RESULTS:
- Gene 8
- Original Glimmer call @bp 6224 has strength 11.15
- SSC:6224.6583 CP:yes SCS:both ST: BLAST-Start: Minl,40, function unknown, 60%, Phages DB GAP: LO: RBS:Kibler7, Karlin Medium, 3.067, -2.451, no
- Gene 8
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- Gene 9
- Original Glimmer call @bp 6552 has strength 13.86
- SSC:6552.7511 CP:yes SCS:both ST: BLAST-Start: Aligns With Ryan, 10, q17:s1, 52%, phages DB GAP: overlap, 329bp LO:yes RBS:Kibler 7, Karlin Medium, 1.145,-6.385, no
- Gene 9
- CONCLUSION:
- Genes 8 and 9 were annotated
- FUTURE STEPS:
- Continue to work on learning how to annotate, and continue to annotate ore of Elesar’s genes