Rationale: The purpose of the lab was to learn more about BLAST and DNA Master and to start annotating gene 1 of Elesar.  

Procedure/ Tools: 

1. DNA Master was opened and Elesar was auto annotated.  
2. The ORF Frames of Elesar were produced and the start codon of gene 1 was changed.  
3. The protein sequence of Elesar gene 1 used to BLAST on NCBI.  
4. Elesar gene 1 was partially auto annotated until BLAST-Start.  
5. The auto annotations were saved to be continued next time.   

Results: 

The partial auto annotation of Elesar gene 1 was SSC 45,353: CP yes: SCS both-cs: ST: BLAST-Start no significant BLAST alignments.: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn

The Start codon of gene 1 was elongated to 45.  

ORF’s of Elesar and RBS results of Gene 1

Conclusion: 

A better understanding was acquired about Blast and DNA Master. Elesar’s gene 1 protein sequence was inserted into NCBI to be compared to other genes, and the results showed no similar genes to found in the database.   

Future Work:  

In the future, gene 1’s annotation will be finished, and a detailed understanding of DNA Master and auto annotating will be obtained.