Gene 32 and Gene 41 Annotation

Rationale: Finish the annotations of Elesar gene 1 and begin Annotations for gene 32 and gene 41

Procedure:

• DNA Master was opened and Elesar FASTA file opened
• Reading frames were opened and genes 32 and 41 were observed
• Both genes were blasted through NCBI Protein Database
• Elesar gene was pulled up on Genemark
• SSC: CP: SCS: BLAST-Start: Gap: LO: RBS were the categories that were annotated

Results:

Blast results shown above for gene 32 and 41 are both from the NCBI Protein database. The images above also show results for each query of the two genes.

Gene 32

SSC: 23223 26093 CP: yes SCS: Both-Both Blast-Start: hypothetical protein PBI_BRIDGETTE, 30, NCBI, Q601: S62, 81%, 0.0 Gap: 7 LO: yes RBS: Kibler 7, Karlin Medium, 2.117, 4.852, no

Gene 41

SSC: 31001 31219 CP: yes SCS: Both-Both Blast-Start: Mycobacterium virus Brujita, 35, NCBI, Q7: S2, 60%, 4e-13 Gap: 607 LO: yes RBS: Kibler 7, Karlin Medium, -6.128, -8.014, no

Conclusions:

Annotations of genes 32 and 41 were not bad after the TA of the class showed the class what each letter meant under the annotation key. Observing the blast was easy while observing genemark results were hard, but with the help of the lab TA’s, it was possible.

Next Steps:

Start annotations for NapoleonB.