Elesar Gene Annotations 1/30/19
Rationale: Annotated genes 6 and 7 of elesar
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Gene 6
SSC: 3685-4974
CP: yes
SCS: both
ST: NA BLAST-Start: matches with Nandita, 7, phagesdb, q1:s1, 97%, 0.0
Gap: 16
LO: yes
RBS: Kibler7, Karlin Medium, 3.215, -2.059, yes
F: portal protein SIF-BLAST: portal protein, phagesdb, Nandita_7, MH834621, 97%, 0.0
SIF-HHPred:
SIF-Syn -
Gene 7
SSC: 5025-6221
CP: yes
SCS: neither
ST: NA
BLAST-Start: matches with Nandita, 8, phagesdb, q1:s14, 93%, 0.0
Gap: 50
LO: no
RBS: Kibler7, Karlin Medium, 1.823, -6.049, no
F: capsid maturation protease
SIF-BLAST: capsid maturation protease, phagesdb, Nandita_8, MH834621, 93%, 0.0
SIF-HHPred:
SIF-Syn
Results/Conclusion:
Annotated 2 genes. Gene 6 I determined to be an RBS because it had the best values for Z score and F score. I changed the starting codon on gene 7 based on the ORF map suggested value and concluded that it was not an RBS.
Future Steps: Figure out if my annotations were correct and figure out how to successfully use template notes.