Elesar Gene 54 & 56 (1/30/19)
Rationale: Finish going over genome annotation temple and practice annotating with Elesar gene 54 and 56.
Tools:
- DNA Master
- NCBI
- PhagesDB
- GeneMark
Procedure:
- The BLAST-Start, Gap, LO, and RBS portions of Elesar gene 1 were filled out.
- BLAST was performed on Elesar gene 54 through both NCBI and PhagesDB.
- Annotated Elesar gene 54.
- BLAST was performed on Elesar gene 56 through both NCBI and PhagesDB.
- Annotated Elesar gene 56.
- These annotations were saved.
Results:
The following image shows Elesar gene 1 fully annotated.
The following images show the GeneMark for Elesar between 36000 to 40000 bp.
The following images show the BLAST results for Elesar gene 54.
The following image shows Elesar gene 54 fully annotated.
The following images show the BLAST results for Elesar gene 56.
The following image shows Elesar gene 56 fully annotated.
Conclusion:
For both Elesar genes 54 and 56, they were most similar to another gene from Elesar. PhagesDB seems to have more BLAST results with smaller E values. Although PhagesDB is a smaller database, it seems to be more helpful than the NCBI database since it has more phage DNA.
Future Work:
Continue to practice annotating with Elesar and learn about phamerator.
