Rationale: BLAST elesar fasta file

Process

1. auto-annotated again
   1. genome -> auto-annotate -> annotate
   2. auto-annotate with pecaan
      1. entered phage name “elesar”
      2. uploaded fasta file
      3. clicked “process”
   3. copied pecaan documentation, pasted it into documentation tab of dna master
   4. removed documentation previously there
   5. clicked “parse”, once directed to a new window, clicked the new “parse” button.
2. Viewed ORFS
   1. DNA -> frames
      1. ORFS button in right hand corner
3. Learned how to change start codon
   1. changed start codon of gene 1 from 84 to 45
4. BLAST gene with DNA Master
   1. select gene
      1. blast tab
      2. blast this gene
      3. wait for BLAST
      4. save to database tab
         1. select E value
         2. save to database

Results/Conclusion:

I could view the different ORFS and evaluate the best starting codon.

Future Steps: learn how annotate a gene