30 January 2019 ✷ DNA Master + BLAST

Procedure

• The FASTa file for Elesar was opened and auto-annotated.
• The “features” tab was opened and within the “notes” box, the code for genes 2 and 3 were annotated.
• The “frame” was pulled up and the ORF was viewed to see if it could be elongated. The start and stop coordinates and gap/overlap were looked at.
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• The genes were each blasted with phagesdb in order to find a similar gene. This information was included in the annotation.
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Results

the annotations for each gene are as follows:

Gene 2

SSC:[346,690] CP:[yes] SCS:[both] ST: BLAST-Start:[Andrew, 1, phagesdb, 1:1, 62%, 3e-17 ] Gap:[8bp overlap] LO:[no] RBS:[Kibler7, Karlin Meduim, 3.167, -2.177, no]

Gene 3

SSC:[954,1352] CP:[yes] SCS:[both] ST: BLAST-Start:[Ryan_4, phagesdb, 1:1, 6e-70, 100%] Gap:[263bp gap] LO:[yes] RBS:[Kibler7, Karlin Medium, 2.573, -3.709, no]

Conclusion

Annotation is useful in research in comparing different genes within differing species that potentially contain the same gene. Practicing this specific skill will be important because the process was somewhat confusing, but it is surely an important method for genetic research.

Future plans

This same method will be applied to Napoleon B, a phage isolated in 2018, in order to analyze its genome.