Rationale

Today we will learn more about the BLAST feature in DNA Master by BLASTing genes in the Elesar genome.

Procedure

• Elesar was opened again in DNA Master and the FASTA file was autoannotated.
• Gene 1 of Elesar was auto-annotated using the details from the annotation key and auto-annotation page.
• The protein sequence of Gene 1 was copied and inserted into the protein BLAST at NCBI.
• The BLAST results were then interpreted.

Results

Blast results are inserted above for Gene 1. Hypothetical protein A of Arthrobacter sp Hiyo is shown to have the highest score. A gap of two is indicated as well.

Conclusions/Next Steps 

A portion of the annotation of Gene 1 was done as a group and will be finished by the next lab. The Gene annotation of Elesar will continue until a better understanding of DNA Master and Blast is reached.