01/30/19

Rationale:

to learn genetic annotation on DNA master.

Procedure:

1. open  DNA master
2. Open FastA file for elesar using the following steps, File>open>FastA multiple sequence file> Choose Elesar.FastA
3. click export on window with the extracted file and then choose Create sequence from this entry only.
4.  to see the frames, go to DNA> Frames.
5. then click ORF on the new window to see the highlighted genes.
6. Click on the BLAST tab and then choose BLAST this gene.
7. zoom in on gene one in the frames window and highlight gene length so that it is as long as possible.
8. lengthen the gene length by changing the starting base pair number of the gene.
9. press the calculator icon and then click post.
10. Using the annotation key and the BLAST results, finish the description of gene 1 as the template requires.
11. repeat the same steps for gene 14 and 15.
12. click post to save changes every time data is altered.
13. save the file.

Results:

Gene 1:SSC:45-353 CP: yes SCS:Both-CS BLAST-Start:no significant BLAST alignment Gap: first gene LO:yes RBS:Kibler7,Karlin medium,1.222,-6.751,no

Gene 14:SSC:8837-9250 CP: yes SCS: both BLAST-Start: Arthrobacter sp. ok362, na, NCBI, Query 14 to subject 1,100%, 0.0E0 Gap:4 LO: yes RBS: Kibler7, Karlin Medium,2.801, -3.226, no

Gene 15:SSC:9307-9969 CP: yes SCS: both BLAST-Start: Arthrobacter phage sp. Hiyo6, 05270,NCBI, Query15 to Subject1,100%,4e-97 Gap:56 LO: yes RBS: Kipler7, Karlin medium,2.801, -3.226, no

Conclusion:

None of the above genes 14 and 15 had significant matches but did not have low  final scores. These two genes have matches with a close to 0 e value.

Future Steps:

continue practice with DNA Master and gene annotation.