01/28/19

Rationale:

to begin learning how to annotate a genome

Procedure:

1. open  DNA master
2. check the “insert template into notes during Auto Annotation” box. Then Apply>OK
3. Open FastA file for elesar using the following steps, File>open>FastA multiple sequence file> Choose Elesar.FastA
4. click export on window with the extracted file and then choose Create sequence from this entry only.
5.  to see the frames, go to DNA> Frames.
6. then click ORF on the new window to see the highlighted genes.
7. Click on the BLAST tab and then choose BLAST this gene.
8. zoom in on gene one in the frames window and highlight gene length so that it is as long as possible.
9. lengthen the gene length by changing the starting base pair number of the gene.
10. press the calculator icon and then click post.
11. Using the annotation key and the BLAST results, fill in the description of the gene for start/stop coordinates and coding potential.
12. click post to save changes.
13. save the file.

Results:

the gene annotation of gene 1 yielded the following: SSC:[45-353]CP: yes SCS:Both-CS

Conclusion:

the BLAST results yielded no significant gene alignment. there is currently no determinable function of this gene.

Future Steps:

complete the annotation of gene 1 and continue annotation.